Superovulatory Response with Injection of Fsh-p in Crossbred Cows: Superovulatory Response with Daily Single Injection of Fsh-p in Polyvenilpyrrolidone Vehicle in Crossbred Cows

The study was conducted on crossbred cows under the ICAR-ETT project, Post-graduate Institute, Department of Animal Reproduction and Gynaecology Dr. PDKV, Akola. Presynchronization of oestrus was done in cows by PGF2 alpha (Lutalyse) 25 mg administered im. Artificial insemination with frozen semen of progeny tested bull (H. F. x Gir 50 per cent) was carried on at an interval of 12 h. Flushing of the donor cows for embryo recovery was carried out on day 7 after last insemination. The time required for onset of superovulatory oestrus from last PGF2 alpha ranged from 38 to 44 h. with an average of 38.66 SE 1.73 h. The length of superovulatory oestrus ranged from 16 to 36 h. with an average of 30 SE 4.76 h. The average number of corpora lutea and unovulatory follicles in the donor cows was 12 SE 5.5 and 2.75 SE 0.85 respectively. The average number of corpora lutea and unovulatory follicles on right and left ovary recorded was 6.5 SE 2.95, 5.5 SE 2.62 and 1.75 SE 0.47; 1.0 SE 0.40, respectively. Total number of embryos recovered from the donor cows was 13 with an average of 3.25 SE 2.01 for each donor cow, out of which 6 embryos were with arrested growth and 7 were degenerated ones.